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:: Volume 1, Issue 1 (April 2017) ::
J Health Sci Technol 2017, 1(1): 11-16 Back to browse issues page
Analysis of Antibiotic Susceptibility Profile and RAPD Typing of Listeria Monocytogenes Isolates
Tayebeh Zeinali , Abdolah Jamshidi * , Mehrnaz Rad , Mohammadreza Bassami
Department of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran , ajamshid@um.ac.ir
Abstract:   (665 Views)

Background: The aim of the present study was to analyze antibiotic susceptibility profileand genetic diversity of L. monocytogenes, isolated from chicken carcasses using RAPD-PCR.

Methods: In this study, 26 isolates of Listeria monocytogenes recovered from chicken carcasses were used. Antibiotic susceptibility of these isolates were examined for eleven antimicrobial agents and RAPD-PCR with use of three different primers were applied to detect genetic diversity of the isolates.

Results: Some of the isolates showed multidrug resistance to commonly used antibiotics. Totally, 26 L. monocytogenes isolates had 16 different antibiogram patterns. Isolates of A and B clusters showed 7 and 6 different pattern, respectively. 12 Isolates of cluster C showed 7 different pattern.

Conclusions: Some of isolates had multidrug resistance to antibiotics. This may raise a public health hazard. RAPD-PCR showed that L. monocytogenes isolated from chicken carcasses had low genetic diversity and there was no clonalrelationship between the isolates.

Keywords: L. monocytogenes, antibiotic resistance, RAPD-PCR
Full-Text [PDF 271 kb]   (335 Downloads)    
Type of Study: Original Article | Subject: Health and food safety and hygiene
Received: 2017/02/5 | Accepted: 2017/03/13 | Published: 2017/04/6
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Zeinali T, Jamshidi A, Rad M, Bassami M. Analysis of Antibiotic Susceptibility Profile and RAPD Typing of Listeria Monocytogenes Isolates. J Health Sci Technol. 2017; 1 (1) :11-16
URL: http://jhst.bums.ac.ir/article-1-21-en.html

Volume 1, Issue 1 (April 2017) Back to browse issues page
مجله علوم و تکنولوژی بهداشت Journal of Health Sciences and Technology
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